BIOL 413: PARASITOLOGY
Course Description
Fall 2017
Updated November 13, 2017  

Dr. Stan Eisen, Director
Preprofessional Health Programs
Biology Department
Christian Brothers University
650 East Parkway South
Memphis, TN  38104
901-321-3447
e-mail:  seisen@cbu.edu

 

 

 

 

BTW, I just found a video of pole dancing instruction that is tasteful and modest...to the extent that pole dancing can be tasteful and modest— 

https://m.youtube.com/watch?v=gO17hN-YvBc

 

Doesn’t this look like Phthirus pubis engaging in pole dancing?

http://www.nejm.org/doi/full/10.1056/NEJMicm1700352

 

Care to hitch a ride on the food chain?

http://www.bonappetit.com/trends/article/15-raw-meat-dishes-from-around-the-world

Phthiriasis…of the eyelashes.(!) 

http://www.youtube.com/watch?feature=player_embedded&v=77TpN2wWBtE

Details about the lecture
Details about the lab
Supplemental reading list
Lecture schedule
Lab schedule

Dr. Stan Eisen
Office S203B
Tel. Ext.: 3447
e-mail: seisen@cbu.edu

Office Hours:       
                              M:  11 a.m.-12 p.m., 1-5 p.m.
                              W: 11 a.m.-12 p.m
                              Th:  9-12 p.m.
                                F:  11 a.m.-12 p.m.; 1-3 p.m.
                                                         
                              

For both lecture and lab, you will need the following:  
          Despommier, et. al.  (2017).  Parasitic Diseases, 6th Edition. 

ISBN :978-0-9978400-0-1 (Hardcover)

ISBN: 978-0-9978400-1-8 (PDF version)

ISBN :978-0-9978400-2-5 (Kindle version)
ISBN: 978-0-9978400-3-2 (iBook version)
        

Eisen, S. (2013). Parasites on Parade. Available from Amazon.com -- http://www.amazon.com/Parasites-Parade-Dr-Stan-Eisen/dp/1484151119/

Some useful resources:

NYU Virtual Microscope BETA 5 : Parasitology

http://cloud.med.nyu.edu/virtualmicroscope/program/2

 

The home page for the website is here:

http://cloud.med.nyu.edu/virtualmicroscope/

 

Enhanced virtual microscopy for collaborative education

BMC Medical Education 2011, 11:4

http://www.biomedcentral.com/content/pdf/1472-6920-11-4.pdf

 

Drisdelle, Rosemary (2010).  Parasites:  Tales of Humanity’s Most Unwelcome Guests. 

In this course, we will be concentrating on the biology of those parasitic organisms which are of medical or veterinary importance. The emphasis of the course will be on natural history, so you will be responsible for the following information pertaining to each parasitic organism covered in class: 1)Binomial nomenclature; 2)Phylogenetic relationship; 3)Life cycle; 4) Preferred definitive host(s); 5)Intermediate and/or vector host(s); 6)Geographical locations where the parasite is found and is endemic; 7)Organs affected; 8)Symptoms associated with infection; and 9)Drugs used in treatment.

DETAILS ABOUT THE LECTURE

Your lecture grade will be based on the following:

1.   Weekly quizzes lasting (maybe) 10 minutes, usually on Fridays, covering the previous week’s lecture material:  These quizzes will be multiple-choice, matching, and short answer.  Cumulatively, they will count 1/6 towards your final grade;

2.   FOUR MIDSEMESTER EXAMS: Each of these exams will consist of essay and identification/practical questions. Each of these exams will be comprehensive and count 1/6 towards your final grade;

3.     A final exam scheduled during Final Exam Week. This exam will count 1/6 towards your final grade. 

DETAILS ABOUT THE LAB

You will be learning techniques which are pertinent to the study of parasitology. These include and will not be limited to the following:

1.     Preparation of permanent slides of stained helminths;

2.     Preparation of wet mount of fecal smears;

3.     Removal of parasitic organisms from dissected host animals;

4.     Concentration of ova by fecal flotation;

5.     Preparation of a smear of parasitic protozoa from earthworms.

The collection of data is a cooperative effort, in which the class will analyze data collected by the entire class.  There are two particular experiments which I would like you to write up as lab reports:  a mosquito bioassay, in which you test the efficacy of at least two larvicides, and a survey of parasites among the centrarchids (sunfish) of Beaver Lake, one of the impoundments located at Shelby Farms.   

With the possible of exception of Giardia lamblia, you will NOT be exposed to human pathogens. Nonetheless, you will be required to follow rigorous safety procedures.

Your final grade will be based on the following:

1.     Weekly quizzes of short answer and identification questions, covering the previous lab’s specimens.  Collectively, these quizzes will count 1/5 towards your final grade;

2.     Two lab reports based on data collected by the entire class.  These experiments will be cooperative laboratory experiences, in which all members of the class will collect data.  You have the option of submitting a group paper, in which a group consists of 2 to 4 students, or you can submit a paper written solo.  The written papers are to be written in the same format for articles appearing in the Transactions of the Tennessee Academy of Science. Grading of the laboratory reports will be on the basis of spelling, grammar, clarity, and content, whereas grading of the oral presentation will be based on clarity, accuracy, and completeness of analysis.   Each report will count 1/5 towards your final grade;

3.     A midterm exam consisting of short answer and identification questions.  This exam will count 1/5 towards your final grade;

4.     A final exam consisting of short answer and identification questions.  This final exam will count 1/5 towards your final grade.

Some of the experiments will require more time than a single four-hour block of time allows. You will be expected to collaborate in order to complete the work. Furthermore, participation in all field trips is mandatory.

The grading scale for the lecture and the lab will be the following:

A = 3.5 - 4.0
B = 2.75 - 3.49
C = 2.00 - 2.74
D = 1.00 - 1.99
F = <1

By the way, regarding laboratory reports:

scrooge

 

 

“Brevity is the soul of wit.”

 

CLASS ATTENDANCE POLICY

Every student is expected to attend classroom and laboratory periods regularly. A student who has been absent, even for a legitimate cause, is under obligation to make up the work. Any student who has missed a total of eight hours of class may be dropped from the course, with a mark of "F", at the discretion of the teacher.

Biol 413: Parasitology Lecture
Fall 2017
Lecture and Lab Schedule

Date

Day

Lecture Topic (Asterisks indicate a Moodle resource)

Textbook Chapter(s)

Parasites on Parade Pages

Lab Schedule (Sequence of labs subject to change, depending on availability of organisms, and on weather for field studies.)

8/21

M

 The magnitude of the problem:

·         A world view*

III.  Eukaryotic parasites

1-13

Tour of Mosquito Control Board facility

Insects:
Order Diptera:  pp. 154-160
Culex spp.

·         Ova

·         Larvae

·         Pupae

·         Adult males

·         Adult females

 

 

 

 

 

 

8/23

W

Introduction:

·         Types of interspecific interactions*
An example of mutualism:  Cell Biology of Cnidarian-Dinoflagellate Symbiosis*

·         Some definitions*

·         Adaptations to a parasitic existence*

·         Candidates for parasitic infections*

·         Six essential aspects of parasite life cycles*

·         Diagnostic methods*

Life cycle of Babesia microti
http://www.youtube.com/watch?v=JOakxoorjoQ

8/25

F

An introduction to the immune system:

·         ComponentsOfImmuneSystem* 

Basic Principles in Immunology & Pathology
http://www.niaid.nih.gov/final/immun/immun.htm

 

8/28

M

Initiate Mosquito bioassay experiment:  pp. 231-234

 

 

 

Studies in Mutualism:  pp. 203-206

Insects which induce gall formation in plants:  pp. 244-246

 

 

Phylum Arthropoda

Class Insecta

Order Anoplura:  pp. 168-174

Pediculus humanus (body louse)

Phthirus pubis (pubic louse)

 

 

 

8/30

W

Phylum Arthropoda: Form, Function and Classification*

 

VIII.  pp. 449-450



149-181

9/1

F

Class Insecta*

Insect development
http://www.cals.ncsu.edu/course/ent425/tutorial/morphogenesis.html

Orders Mallophaga & Anoplura*

·         Pediculus humanus*

http://www.youtube.com/watch?v=5a3_SZtgHYE

·         Phthirus pubis*

·         -- on eyelashes:

Phthiriasis

http://www.youtube.com/watch?v=TpKvYtLxvvI

Phthiriasis treatment, 1 week later:

https://www.youtube.com/watch?v=EFhdFzJcs00

 

38

 

 

 

9/4

M

Labor Day – No class

 

 

 

Order Hemiptera:  pp. 161-163

Cimex lectularis (bedbug)

·         Adults, w.m.

 

Order Siphonoptera:164-167

Pulex irritans (human flea)

Ctenocephalides canis (dog flea)

·         Adults, w.m.

9/6

W

Hemiptera: True bugs*

·         Cimex lectularis*

·         Rhodnius prolixus*

Triatoma infestans*

38

 

 

 

38

 

9/8

F

Siphonoptera: The fleas*

·         Pulex irritans*

·         Ctenocephalides spp.*

·         Xenopsylla cheopis*

·         Tunga penetrans*

What’s causing that itch?  (A slideshow from Medscape):
http://www.medscape.com/features/slideshow/bedbugs-lice-mites?src=mp&spon=17&uac=40240FX

38

164-167

9/11

M

Diptera: Family Culicidae (Mosquitos)*

http://facstaff.cbu.edu/~seisen/TheZikaFile.htm

Diptera: Other flies

·         Simulium spp.*

·         Glossina spp.*

·         Lutzomyia spp.*

·         Chrysops spp.*

·         Phlebotomus spp.*

·         Botflies:  http://ambergriscaye.com/pages/town/botfly.html

·         Cuterebra:  http://entnemdept.ufl.edu/creatures/misc/flies/squirrel_bot_fly.htm

 

38

 

 

 

 

 

 

 

154-160

Order Diptera, continued

Glossina spp.

·         Adults

Simulium spp.

·         Adults

Botflies

·         Larvae

Order Hymenoptera

·         Melittobia digitata adults emerging from blowfly larvae; preparation of semi-permanent slides

 

9/13

W

Hymenoptera*

·         Copidosoma floridanum*

·         Infected caterpillar becomes a “nursery” for parasitic wasps:  http://www.youtube.com/watch?v=vMG-LWyNcAs&feature=related

·         The wasp Ampulex compressa is a parasitoid, whose females inject venom to lay an egg into the cockroach Periplaneta americana -- “Zombie” cockroach -- http://www.youtube.com/watch?v=DzGCSk1Zpoo

·         Jewel wasp paralyzing a cockroach, laying its egg, and barricading it in a laboratory setting:  http://www.youtube.com/watch?v=qN2XMyxAs5o 

Cicada-killer trying to subdue a cicada:
http://www.youtube.com/watch?v=8yjrwkTpAtc

38

 

9/15

F

LECTURE EXAM I

 

 

9/18

M

Parasitic Arachnids: Ticks and Mites

Films on Demand from the Library

Lyme Disease, the Silent Epidemic

http://libproxy.cbu.edu/login?url=http://fod.infobase.com/PortalPlaylists.aspx?wID=104145&xtid=58383

"Everything you never wanted to know about the mites that eat, crawl, and have sex on your face" -- http://blogs.discovermagazine.com/notrocketscience/2012/08/31/everything-you-never-wanted-to-know-about-the-mites-that-eat-crawl-and-have-sex-on-your-face/

·         Dermacentor andersoni*

·         Ixodes spp.*

·         Trombicula alfreddugesi*

·         Demodex spp. -- http://www.youtube.com/watch?v=4IoWb2bUqmk

·         Sarcoptes scabiei*

39

 

 

 

 

 

 

175-181

 

 

 

 

 

 

Dermacentor andersoni

·         Larvae

·         Nymphs

·         Adult females

·         Adult males

Ixodes spp.

·         Adults

Family Trombiculidae

·         Adult from Rana pipiens

Class Digenea:  pp. 56-76

Schistosoma spp.

Schistosoma mansoni

·         Adult male, w.m.

·         Adult female, w.m.

·         Ova in fecal smear, w.m.

·         Ova in liver tissue, c.s.

·         Miracidia, w.m.

·         Cercariae, w.m.

Schistosoma haematobium

·         Ova in fecal smear, w.m.

Schistosoma japonicum

·         Ova in fecal smear, w.m.

·         Adult male, w.m.

·         Adult female, w.m.

 

 

9/20

W

Phylum Platyhelminthes*

Trematoda: Form, Function, and Classification of Digeneans

Digeneans: Strigeiformes (The schistosomes*)

Schistosomiasis (1990): Entire title:

https://www.youtube.com/watch?v=EvbkdpHwOKk

Living with Schistosomiasis:

https://www.youtube.com/watch?v=5wQbpgAdIMs

Granuloma Formation by Schistosoma*

VII:  pp. 389-390

33

 

54-56

 

9/22

F

Learning Module I:  An Introduction To Epidemiology*

 

 

9/25

M

Digeneans (Comparison of “typical” digenean, Clonorchis sinensis, with the “atypical” Schistosoma genus*):

·         Fasciola hepatica*  

·         Fasciolopsis buski* 

·          Paragonimus westermani* 

·         Clonorchis sinensis*

 

34 - 37

56-77


Paragonimus westermanni

·         Adult, w.m.

·         Ova in fecal smear, w.m.

·         Metacercariae, w.m.


Clonorchis sinensis

·         Adult, w.m.

·         Adult in infected liver, c.s.

·         Ova in fecal smear, c.s.

·         Adults, c.s.


Fasciola hepatica

·         Adult, w.m.

·         Ova in fecal smear, w.m.

·         Miracidia, w.m.

·         Redia, w.m.

·         Cercariae, w.m.

Fasciolopsis buski

·         Adult, w.m.

·         Incubated ova, w.m.

·         Ova in fecal smear, w.m.

9/27

W

Digeneans, continued

 

 

9/29

F

Cestoidea:  Form, Function, and the Classification of the Tapeworms*

Tapeworms: 

·         Pseudophyllidea

·                  Diphllobothrium latum*  

VI.  pp. 329-330

 

30

56-77

10/2

M

·         Cylophyllidea

·                 Taenia solium* 

·                 Taeniarhynchus saginata*

·                  Echinococcus granulosus* 

·                  Hymenolepis diminuta*

·                  Vampirolepis nana* 

·                  Dipylidium caninum* 

28-32

78-100

 

 

Class Cestoda: pp. 77-101.

Hymenolepis diminuta

·         Adult, w.m.

·         Ova in fecal smear

·         Cysticercoid larva, w.m.

Diphyllobothrium latum

·         Scolex, w.m.

·         Mature proglottid, w.m.

·         Gravid proglottid, w.m.

·         Plerocercoid larva, w.m.

·         Ova in fecal smear

Echinococcus granulosus

·         Adult, w.m.

·         Ova in fecal smear

·         Cysticercus larva, w.m.

·         Hydatid cyst, c.s.

·         Hydatid sand, w.m.

Dipylidium caninum

·         Adult, composite, w.m.

Ova in fecal smear

10/4

W

Cyclophyllidea continued

 

 

10/6

F

Module II

 

 

10/9

M

Lecture Exam 2

 

 

Intensity, incidence and frequency distributions of parasites collected from fishes of Beaver Lake, Shelby Farms, Memphis:  pp. 203-208; 209-215.

10/12

W

Lab Midterm Exam given during lecture period

 

 

10/14

F

Module III

 

 

10/16-10/20

M-F

FALL BREAK (Sukkot occur during Fall Break)

 

 

 

10/23

M

Analysis of data collected from centrarchids of Beaver Lake

 

 

Phylum Nematoda:  pp. 101-132.


Strongyloides stercoralis

·         Free-living forms

·         Parasitic larvae

 

Ascaris lumbricoides

·         Ova

·         Adult females, whole

·         Adult males, whole

·         Adult females, c.s.

·         Adult males, c.s.

Toxocara spp.

·         Adult males and females

·         Ova

Nematodes:
Trichuris trichiura

·         Adults

·         Ova

Trichinella spiralis

·         3rd stage larvae in muscle tissue

·         Adult females and males

10/25

W

Phylum Nematoda*  

Nematoda:  Trichurida and Dioctophymatida

·         Trichuris trichiura*

·         Trichinella spiralis*

Nematoda:  Rhabditida, Pioneering Parasites

·         Strongyloides stercoralis

 

V. pp. 199-200

 

 

 

17, 21

 

 

 

20

 

 

101-132

10/27

F

The Hookworms

·         Necator americanus

·         Ancylostoma duodenale

·         Ancylostoma ceylanicum

·         Ancylostoma caninum

Ascaris lumbricoides

19

 

 

 

18

 

 

 

 

 

 

 

 

 

 

141-146

10/30

M

Enterobius vermicularis

Nematoda:  Spirurida, a Potpourri

Nematodes:  (Filaroidea), including Wuchereria, Onchocerca and Dirofilaria

·         Wuchereria bancroft

·         Onchocerca volvulus*

·         Loa loa

·         Dirofilaria immitis 

Nematodes, the Guinea Worms & Others

Dracunculus medinensis* 

 

 

 

16

22-24

 

 

 

 

25, 26

 

 

 

 

Phylum Nematoda