BIOL 413: PARASITOLOGY
Course Description
Fall 2015
Updated August 14, 2015  

Dr. Stan Eisen, Director
Preprofessional Health Programs
Biology Department
Christian Brothers University
650 East Parkway South
Memphis, TN  38104
901-321-3447
e-mail:  seisen@cbu.edu

Care to hitch a ride on the food chain?

http://www.bonappetit.com/trends/article/15-raw-meat-dishes-from-around-the-world

http://www.youtube.com/watch?feature=player_embedded&v=77TpN2wWBtE

Details about the lecture
Details about the lab
Supplemental reading list
Lecture schedule
Lab schedule

Dr. Stan Eisen
Office S203B
Tel. Ext.: 3447
e-mail: seisen@cbu.edu

Office Hours:       
                              M:  9 a.m. to 10 a.m., 1 to 5 p.m.
                             T:  1 to 5 p.m.
                                 Th:  9 a.m. to 12 p.m.
                                 F:  9 a.m. to 10 a.m.

Some useful resources:

NYU Virtual Microscope BETA 5 : Parasitology

http://cloud.med.nyu.edu/virtualmicroscope/program/2

 

The home page for the website is here:

http://cloud.med.nyu.edu/virtualmicroscope/

 

Enhanced virtual microscopy for collaborative education

BMC Medical Education 2011, 11:4

http://www.biomedcentral.com/content/pdf/1472-6920-11-4.pdf

 

Drisdelle, Rosemary (2010).  Parasites:  Tales of Humanity’s Most Unwelcome Guests. 

In this course, we will be concentrating on the biology of those parasitic organisms which are of medical or veterinary importance. The emphasis of the course will be on natural history, so you will be responsible for the following information pertaining to each parasitic organism covered in class: 1)Binomial nomenclature; 2)Phylogenetic relationship; 3)Life cycle; 4) Preferred definitive host(s); 5)Intermediate and/or vector host(s); 6)Geographical locations where the parasite is found and is endemic; 7)Organs affected; 8)Symptoms associated with infection; and 9)Drugs used in treatment.

DETAILS ABOUT THE LECTURE

Your lecture grade will be based on the following:

1.   Weekly quizzes lasting (maybe) 10 minutes, usually on Fridays, covering the previous week’s lecture material:  These quizzes will be multiple-choice, matching, and short answer.  Cumulatively, they will count 1/8 towards your final grade;

2.   FOUR MIDSEMESTER EXAMS: Each of these exams will consist of essay and identification/practical questions. Each of these exams will be comprehensive and count 1/8 towards your final grade;

3.     An oral presentation on an assigned topic relevant to the course:  The class will be divided into groups, and each group will be responsible for giving a presentation on the major points presented in their assigned chapters.  Each group is responsible for submitting an outline of their presentation AND 5 multiple-choice questions (with answers) at least 24 hours in advance of their presentation.  This presentation, and accompanying outline and questions, will count 1/8 towards your final grade;

4.     A final exam scheduled during Final Exam Week. This exam will count 2/8, or 1/4 towards your final grade. 

Regarding the oral presentations, each group MUST:

1)    Make an appointment to meet with me some time in the week prior to their presentation so that we can review the presentation (and PowerPoint file);

2)    Prepare review notes of their presentation and send them to me as .doc or .docx files at least 24 hours in advance so that I can forward them to the rest of the students;

3)    Prepare 5 multiple-choice questions, with the answers CLEARLY indicated, and send them to me as a .doc or .docx file at least 24 hours in advance so that I can forward them to the rest of the students

4)    Give a 15-20 minute presentation on their topic

5)    The grading rubric for the presentation, based on a 4-point scale is fairly simple:

If you:

Then you’ll get:

     Meet with me at least 48 hours in advance to discuss your presentation, and review pronunciation.  Furthermore, it sounds intelligent and thoughtful.

.5 point

     Submit the review notes at least 24 hours in advance so that I can forward them to the class.


.5 point

     Submit 5 multiple-choice questions, WITH ANSWERS INDICATED at least 24 hours in advance, so that I can forward them to the class. 


.5 point

     Show up on your appointed day, awake, lucid & sober


.5 point

     You present information in a logical, interesting, and professional manner, and demonstrate full knowledge of the subject.

 


2 points

 

TOTAL

 

4        points (i.e., the MAX)

Topics for oral presentations from 2014 – subject to change for 2015:

Date

Topic

THE WINNERS!

F 9/11

Presentation 1. How outbreaks of bubonic plague altered European society and culture. 

Daniel Gabriel

Leslie Hogan

W 9/16

Presentation 2:  What’s this about drug-resistant head lice? 

 

M 9/30

Presentation 3:  Drugs used in the treatment of schistosomiasis, and their modes of action.

Cale Alexander

John Buttross

F 10/12

Presentation 4:  The (military) “mission-aborting” potential of parasitic and infectious diseases.   

 

M 11/2

Presentation 5:  Nematode therapy for the treatment of allergies and autoimmune diseases.

 

Katie Robinson

Rachel Depperschmidt

 

W 11/4

Presentation 6:  Ocular issues in parasitology

Chelsea Casachia

Joe Krebs

F 11/6

Presentation 7:  Drugs used in the treatment of intestinal nematodes and their modes of action.

Brent McGlaughlin

Ryan Tomlinson

 

M 11/9

Presentation 8:  Therapeutic use of medicinal leeches in microsurgery.

 

Kyle Fiorinelli

Daniel Schenck

F 11/20

Presentation 9:  Biological mechanism for changing surface antigen expression in African trypanosomes.  

Jacob Mann

 

W 11/25

Presentation 10:  Epidemiology of water-borne diseases caused by intestinal protozoa, e.g. Cryptosporidium parvum, Giardia lamblia, and Entamoeba histolytica

Camisha Terrell

Bailey Smith

 

Presentation 11:  Incidence of STDs in Shelby County, treatment and prevention. 

Keyara Baltimore

Daria Dyer

M 11/30

Presentation 12:  Drugs used for the treatment of intestinal protozoa, and their modes of action. 

Jacob Beaty

Patrick Gurley

 

 

DETAILS ABOUT THE LAB

You will be learning techniques which are pertinent to the study of parasitology. These include and will not be limited to the following:

1.     Preparation of permanent slides of stained helminths;

2.     Preparation of wet mount of fecal smears;

3.     Removal of parasitic organisms from dissected host animals;

4.     Concentration of ova by fecal flotation;

5.     Preparation of a smear of parasitic protozoa from earthworms.

These techniques will, in turn, be integrated into 4 experiments, 3 of which you will write up as lab reports.

1.     Survey of parasites collected from freshwater fish collected locally;

2.     Conditions required for the excystation of Posthodiplostomum minimum metacercariae;

3.     Life history and control of mosquitos.

The class will be divided into 4 groups.  Each group will be responsible for the collection and analysis of data for one of the experiments mentioned above, and will give an oral presentation to the entire class and submit a written lab report to me, interpreting the results.   

Generally, you will NOT be exposed to human pathogens. Nonetheless, you will be required to follow rigorous safety procedures.

Your final grade will be based on the following:

1.     Weekly quizzes of short answer and identification questions, covering the previous lab’s specimens.  Collectively, these quizzes will count 1/6 towards your final grade;

2.     Two lab reports based on data collected by the entire class.  These experiments will be cooperative laboratory experiences, in which all members of the class will collect data.  You have the option of submitting a group paper, in which a group consists of 2 to 4 students, or you can submit a paper written solo.  The written papers are to be written in the same format for articles appearing in the Transactions of the Tennessee Academy of Science. Grading of the laboratory reports will be on the basis of spelling, grammar, clarity, and content, whereas grading of the oral presentation will be based on clarity, accuracy, and completeness of analysis.   The paper will count 1/6 towards your final grade;

3.     A midterm exam consisting of short answer and identification questions.  This exam will count 1/6 towards your final grade;

4.     A final exam consisting of short answer and identification questions.  This final exam will count 2/6, or 1/3 towards your final grade.

Some of the experiments will require more time than a single four-hour block of time allows. You will be expected to collaborate in order to complete the work. Furthermore, participation in all field trips is mandatory.

The grading scale for the lecture and the lab will be the following:

A = 3.5 - 4.0
B = 2.75 - 3.49
C = 2.00 - 2.74
D = 1.00 - 1.99
F = <1

By the way, regarding laboratory reports:

scrooge

 

 

“Brevity is the soul of wit.”

 

CLASS ATTENDANCE POLICY

Every student is expected to attend classroom and laboratory periods regularly. A student who has been absent, even for a legitimate cause, is under obligation to make up the work. Any student who has missed a total of eight hours of class may be dropped from the course, with a mark of "F", at the discretion of the teacher.

Biol 413: Parasitology Lecture
Fall 2015
Lecture and Lab Schedule

Date

Day

Lecture Topic (Asterisks indicate a Moodle resource)

Textbook Chapter(s)

Parasites on Parade Pages

Lab Schedule (Sequence of labs subject to change, depending on availability of organisms, and on weather for field studies.)

8/24

M

 The magnitude of the problem:

·         A world view*

1-2

1-13

Studies in Mutualism:  pp. 203-206

Insects which induce gall formation in plants:  pp. 244-246

 

8/26

W

Introduction:

·         Types of interspecific interactions*
An example of mutualism:  Cell Biology of Cnidarian-Dinoflagellate Symbiosis*

·         Some definitions*

·         Adaptations to a parasitic existence*

·         Candidates for parasitic infections*

·         Six essential aspects of parasite life cycles*

·         Diagnostic methods*

Life cycle of Babesia microti
http://www.youtube.com/watch?v=JOakxoorjoQ

8/28

F

An introduction to the immune system:

·         ComponentsOfImmuneSystem* 

Basic Principles in Immunology & Pathology
http://www.niaid.nih.gov/final/immun/immun.htm

3

 

8/31

M

Phylum Arthropoda: Form, Function and Classification*

·         Parasitic crustaceans*

·         Cymothoa exigua is a crustacean which causes the tongue of a fish to atrophy and fall off.(!)*

http://www.youtube.com/watch?v=327-bwMQI-Y&feature=youtu.be

Argulus

Stationary: 

https://www.youtube.com/watch?v=2vPwaDtMYrs 

Swimming:

https://www.youtube.com/watch?v=b1Q-dLgNk2c

Removal: 

https://www.youtube.com/watch?v=ZsromkSwzaY 

 

33


34

149-181

Phylum Arthropoda

Class Crustacea

Argulus sp.

·         Adults

Lernaea sp.

·         Adults

Caligus

·         Adults

 

 

Tour of Mosquito Control Board facility

9/2

W

Class Insecta*

Insect development
http://www.cals.ncsu.edu/course/ent425/tutorial/morphogenesis.html

Orders Mallophaga & Anoplura*

·         Pediculus humanus*

http://www.youtube.com/watch?v=5a3_SZtgHYE

·         Phthirus pubis*

·         -- on eyelashes:

http://www.youtube.com/watch?v=TpKvYtLxvvI

 

33

 

9/4

F

Hemiptera: True bugs*

·         Cimex lectularis*

·         Rhodnius prolixus*

Triatoma infestans*

36

 

 

 

37

 

 

 

9/7

M

Labor Day – No class

 

 

 

Mosquito bioassay experiment:  pp. 231-234

 

Class Insecta

Order Anoplura:  pp. 168-174

Pediculus humanus (body louse)

Phthirus pubis (public louse)

Order Siphonoptera:164-167

Pulex irritans (human flea)

Ctenocephalides canis (dog flea)

·         Adults, w.m.

 

Order Hemiptera:  pp. 161-163

Cimex lectularis (bedbug)

·         Adults, w.m.

9/9

W

Siphonoptera: The flea*s

·         Pulex irritans*

·         Ctenocephalides spp.*

·         Xenopsylla cheopis*

·         Tunga penetrans*

What’s causing that itch?  (A slideshow from Medscape):
http://www.medscape.com/features/slideshow/bedbugs-lice-mites?src=mp&spon=17&uac=40240FX

38

164-167

9/11

F

Diptera: Family Culicidae (Mosquitos)*

Presentation 1:  How outbreaks of bubonic plague altered European society and culture. 

 

Diptera: Other flies

·         Simulium spp.*

·         Glossina spp.*

·         Lutzomyia spp.*

·         Chrysops spp.*

·         Phlebotomus spp.*

·         Botflies:  http://ambergriscaye.com/pages/town/botfly.html

Cuterebra:  http://entnemdept.ufl.edu/creatures/misc/flies/squirrel_bot_fly.htm

39

154-160

9/14

M

Rosh Hashanah I – No classes

 

 

Insects:
Order Diptera:  pp. 154-160
Culex spp.

·         Ova

·         Larvae

·         Pupae

·         Adult males

·         Adult females

Glossina spp.

·         Adults

 

9/16

W

Hymenoptera*

Presentation 2:  Use of Hymenoptera as a biological control of pest insects. 

·         Copidosoma floridanum*

·         Infected caterpillar becomes a “nursery” for parasitic wasps:  http://www.youtube.com/watch?v=vMG-LWyNcAs&feature=related

·         The wasp Ampulex compressa is a parasitoid, whose females inject venom to lay an egg into the cockroach Periplaneta americana -- “Zombie” cockroach -- http://www.youtube.com/watch?v=DzGCSk1Zpoo

·         Jewel wasp paralyzing a cockroach, laying its egg, and barricading it in a laboratory setting:  http://www.youtube.com/watch?v=qN2XMyxAs5o 

·         Cicada-killer trying to subdue a cicada:
http://www.youtube.com/watch?v=8yjrwkTpAtc

40

 

9/18

F

LECTURE EXAM I

 

 

9/21

M

Parasitic Arachnids: Ticks and Mites

"Everything you never wanted to know about the mites that eat, crawl, and have sex on your face" -- http://blogs.discovermagazine.com/notrocketscience/2012/08/31/everything-you-never-wanted-to-know-about-the-mites-that-eat-crawl-and-have-sex-on-your-face/

·         Dermacentor andersoni*

·         Ixodes spp.*

·         Trombicula alfreddugesi*

·         Demodex spp. -- http://www.youtube.com/watch?v=4IoWb2bUqmk

·         Sarcoptes scabiei*

An Introduction To Epidemiology*

41

 

 

 

 

 

4

175-181

 

 

 

 

 

11-119

Arachnids: pp. 175-181.
Dermacentor andersoni

·         Adult females

·         Adult males

·         Larvae

Ixodes spp.

·         Adults

Family Trombiculidae

·         Adult from Rana pipiens

 

 

9/23

W

Yom Kippur – No classes

 

 

9/25

F

Phylum Platyhelminthes*
Aspidobothrea

13
14

 

9/28

M

Sukkot I – No classes

 

 

Class Digenea:  pp. 56-76

Schistosoma spp.

Schistosoma mansoni

·         Adult male, w.m.

·         Adult female, w.m.

·         Ova in fecal smear, w.m.

·         Ova in liver tissue, c.s.

·         Miracidia, w.m.

·         Cercariae, w.m.

Schistosoma haematobium

·         Ova in fecal smear, w.m.

Schistosoma japonicum

·         Ova in fecal smear, w.m.

·         Adult male, w.m.

·         Adult female, w.m.


Paragonimus westermanni

·         Adult, w.m.

·         Ova in fecal smear, w.m.

·         Metacercariae, w.m.


Clonorchis sinensis

·         Adult, w.m.

·         Adult in infected liver, c.s.

·         Ova in fecal smear, c.s.

·         Adults, c.s.


Fasciola hepatica

·         Adult, w.m.

·         Ova in fecal smear, w.m.

·         Miracidia, w.m.

·         Redia, w.m.

·         Cercariae, w.m.

Fasciolopsis buski

·         Adult, w.m.

·         Incubated ova, w.m.

·         Ova in fecal smear, w.m.

 

9/30

W

Trematoda: Form, Function, and Classification of Digeneans

Digeneans: Strigeiformes (The schistosomes*)

Granuloma Formation by Schistosoma*

Presentation 3:  Drugs used in the treatment of schistosomiasis, and their modes of action

13

14

 

15

      16

54-56

 

10/2

F

Digeneans:

·         Fasciola hepatica*  

·         Fasciolopsis buski* 

·          Paragonimus westermani* 

·         Clonorchis sinensis*

Monogenea

Parasites of centrarchids collected from Beaver Lake* (Preparatory to next week’s lab)

17

18

56-77

10/5

M

Shimini Atzeret – No class

 

 

Intensity, incidence and frequency distributions of parasites collected from fishes of Beaver Lake, Shelby Farms, Memphis:  pp. 203-208; 209-215.

10/7

W

Collection and interpretation of parasite distribution data

 

 

10/9

F

 Cestoidea:  Form, Function and Classification of the Tapeworms*

20

21

 

10/12

M

Tapeworms: 

·         Diphllobothrium latum*  

·         Taenia solium* 

·         Taeniarhynchus saginata*  

Presentation 4:  Surveillance and treatment of infectious diseases among zoo animals. 

20

21

78-100

 

 

Class Cestoda: pp. 77-101.

Hymenolepis diminuta

·         Adult, w.m.

·         Ova in fecal smear

·         Cysticercoid larva, w.m.

Diphyllobothrium latum

·         Scolex, w.m.

·         Mature proglottid, w.m.

·         Gravid proglottid, w.m.

·         Plerocercoid larva, w.m.

·         Ova in fecal smear

Echinococcus granulosus

·         Adult, w.m.

·         Ova in fecal smear

·         Cysticercus larva, w.m.

·         Hydatid cyst, c.s.

·         Hydatid sand, w.m.

Dipylidium caninum

·         Adult, composite, w.m.

Ova in fecal smear

10/14

W

Tapeworms, continued:

·         Echinococcus granulosus* 

·         Hymenolepis diminuta*

·         Vampirolepis nana* 

·         Dipylidium caninum* 

 

 

10/16

F

Lecture Exam 2

 

 

10/19-10/25

M-Su

FALL BREAK

 

 

 

10/26

M

Lab Midterm Exam

 

Phylum Nematoda:  pp. 101-132.


Strongyloides stercoralis

·         Free-living forms

·         Parasitic larvae

Phylum Nematoda
Hookworms

·         Ova

·         Necator americanus adult females

·         Necator americans adult males

·         Ancylostoma duodenale adult females

·         Ancylosotma duodenale adult males

·         Ancylostoma caninum adult females

·         Ancylostoma caninum adult males

10/28

W

Phylum Nematoda*  

Nematoda:  Trichurida and Dioctophymatida

·         Trichuris trichiura*  

·         Trichinella spiralis*

22

 

 

23

101-132

 

10/30

F

Nematoda:  Rhabditida, Pioneering Parasites

·         Strongyloides stercoralis

Nematoda:  Strongylidae, Bursate Rhabditians, aka hookworms

·         Ancyclostoma duodenale

·         Ancylostoma caninum

·         Necator americanus

24

 

25

101-132

11/2

M

Nematodes:  Ascaridida, Intestinal Roundworms

Presentation 5:  Nematode therapy for the treatment of allergies and autoimmune diseases.

·         Ascaris lumbricoides 

·         Toxocara

Nematoda:  Oxyurida, the Pinworms

·         Enterobius vermicularis*

 

 

26

27

 

29

 

30

101-132

Ascaris lumbricoides

·         Ova

·         Adult females, whole

·         Adult males, whole

·         Adult females, c.s.

·