BIOL 413: PARASITOLOGY
Course Description
Fall 2012
Updated July 31, 2014  

Dr. Stan Eisen, Director
Preprofessional Health Programs
Biology Department
Christian Brothers University
650 East Parkway South
Memphis, TN  38104
901-321-3447
e-mail:  seisen@cbu.edu

 

http://www.youtube.com/watch?feature=player_embedded&v=77TpN2wWBtE

Details about the lecture
Details about the lab
Supplemental reading list
Lecture schedule
Lab schedule

Dr. Stan Eisen
Office S203B
Tel. Ext.: 3447
e-mail: seisen@cbu.edu

Office Hours:       
                              M:  9 a.m. to 11 noon, 1 to 5 p.m.
                             Th:  1 to 5 p.m.
                                 F: 9 a.m. to 11a.m.; 1 p.m. - 2 p.m.

Some useful resources:

NYU Virtual Microscope BETA 5 : Parasitology

http://cloud.med.nyu.edu/virtualmicroscope/program/2

 

The home page for the website is here:

http://cloud.med.nyu.edu/virtualmicroscope/

 

Enhanced virtual microscopy for collaborative education

BMC Medical Education 2011, 11:4

http://www.biomedcentral.com/content/pdf/1472-6920-11-4.pdf

 

Drisdelle, Rosemary (2010).  Parasites:  Tales of Humanity’s Most Unwelcome Guests. 

In this course, we will be concentrating on the biology of those parasitic organisms which are of medical or veterinary importance. The emphasis of the course will be on natural history, so you will be responsible for the following information pertaining to each parasitic organism covered in class: 1)Binomial nomenclature; 2)Phylogenetic relationship; 3)Life cycle; 4) Preferred definitive host(s); 5)Intermediate and/or vector host(s); 6)Geographical locations where the parasite is found and is endemic; 7)Organs affected; 8)Symptoms associated with infection; and 9)Drugs used in treatment.

DETAILS ABOUT THE LECTURE

Your lecture grade will be based on the following:

1.   FOUR MIDSEMESTER EXAMS: Each of these exams will consist of essay and identification/practical questions. Each of these exams will be comprehensive and count 1/6 towards your final grade;

2.     A presentation on an assigned chapter from Parasite Rex OR on an assigned topic relevant to the course:  The class will be divided into groups, and each group will be responsible for giving a presentation on the major points presented in their assigned chapters.  Each group is responsible for submitting an outline of their presentation AND 5 multiple-choice questions (with answers) at least 24 hours in advance of their presentation.  This presentation, and accompanying outline and questions, will count 1/6 towards your final grade;

Regarding the oral presentations, each group MUST:

1)    Make an appointment to meet with me some time in the week prior to their presentation so that we can review the presentation (and PowerPoint file);

2)    Prepare review notes of their presentation and send them to me as .doc or .docx files at least 24 hours in advance so that I can forward them to the rest of the students;

3)    Prepare 5 multiple-choice questions, with the answers CLEARLY indicated, and send them to me as a .doc or .docx file at least 24 hours in advance so that I can forward them to the rest of the students

4)    Give a 15-30 minute presentation on their topic

5)    The grading rubric for the presentation, based on a 4-point scale is fairly simple:

If you:

Then you’ll get:

     Meet with me to discuss your presentation, and it sounds intelligent


1 point

     Submit the review notes at least 24 hours in advance


.5 point

     Submit 5 multiple-choice questions, WITH ANSWERS INDICATED at least 24 hours in advance


.5 point

     Show up on your appointed day, awake, lucid & sober


.5 point

     Not only do you show up on your appointed day, awake, lucid & sober, your presentation is considered interesting and comprehensible by your peers in the class.

 


1.5 point

 

TOTAL

 

4        points (i.e., the MAX)

Topics for oral presentations:

Topic

Date

THE WINNER!

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3.     A final exam scheduled during Final Exam Week. This exam will count 1/6 towards your final grade. 

 

DETAILS ABOUT THE LAB

You will be learning techniques which are pertinent to the study of parasitology. These include and will not be limited to the following:

1.     Preparation of permanent slides of stained helminths;

2.     Preparation of wet mount of fecal smears;

3.     Removal of parasitic organisms from dissected host animals;

4.     Concentration of ova by fecal flotation;

5.     Preparation of a smear of parasitic protozoa from earthworms.

These techniques will, in turn, be integrated into 4 experiments, 3 of which you will write up as lab reports.

1.     Incidence of helminth ova found in dog fecal samples collected from dog parks in the greater Memphis area.  The relevant results are frequency distributions of parasite incidence and the number of parasite species found per host;

2.     Survey of parasites collected from freshwater fish collected locally;

3.     Conditions required for the excystation of Posthodiplostomum minimum metacercariae;

4.     Life history and control of mosquitos.

The class will be divided into 4 groups.  Each group will be responsible for the collection and analysis of data for one of the experiments mentioned above, and will give an oral presentation to the entire class and submit a written lab report to me, interpreting the results.   

Generally, you will NOT be exposed to human pathogens. Nonetheless, you will be required to follow rigorous safety procedures.

Your final grade will be based on the following:

1.     Weekly quizzes of short answer and identification questions, covering the previous lab’s specimens.  Collectively, these quizzes will count 1/5 towards your final grade;

2.     A written paper of an experiment conducted by the entire class.  These experiments will be cooperative laboratory experiences, in which all members of the class will collect data, but only one group will collect the data for a particular experiment and analyze the data for the written paper.  The written papers are to be written in the same format for articles appearing in the Transactions of the Tennessee Academy of Science and will be due on the last day of classes. Grading of the laboratory reports will be on the basis of spelling, grammar, clarity, and content, whereas grading of the oral presentation will be based on clarity, accuracy, and completeness of analysis.   The paper will count 1/5 towards your final grade.  (N.B.:  The oral presentation was cancelled due to the cancellation of classes on December 6 due to inclement weather.);

3.     A midterm exam consisting of short answer and identification questions.  This exam will count 1/5 towards your final grade;

4.     A final exam consisting of short answer and identification questions.  This final exam will count 2/5 towards your final grade.

Some of the experiments will require more time than a single four-hour block of time allows. You will be expected to collaborate in order to complete the work. Furthermore, participation in all fishing and field trips is mandatory.

The grading scale for the lecture and the lab will be the following:

A = 3.5 - 4.0
B = 2.75 - 3.49
C = 2.00 - 2.74
D = 1.00 - 1.99
F = <1

By the way, regarding laboratory reports:

scrooge

 

 

“Brevity is the soul of wit.”

 

CLASS ATTENDANCE POLICY

Every student is expected to attend classroom and laboratory periods regularly. A student who has been absent, even for a legitimate cause, is under obligation to make up the work. Any student who has missed a total of eight hours of class may be dropped from the course, with a mark of "F", at the discretion of the teacher.

Biol 413: Parasitology Lecture
Fall 2014
Lecture Schedule

Date

Day

Lecture Topic

Textbook Chapter(s)

Parasites on Parade Pages

8/25

M

 The magnitude of the problem:
http://facstaff.cbu.edu/~seisen/DevelopingWorldParasites.htm 

Parasite Video

1-2

1-13

8/27

W

Parasite Video, cont’d

8/29

F

Introduction:

·         Types of interspecific interactions

·         Some definitions

·         Adaptations to a parasitic existence

·         Candidates for parasitic infections

·         Six essential aspects of parasite life cycles

·         Diagnostic methods

·         A world view

Life cycle of Babesia microti
http://www.youtube.com/watch?v=JOakxoorjoQ

 

9/1

M

Labor Day – No class

 

 

9/3

W

An introduction to the immune system:
http://facstaff.cbu.edu/~seisen/ComponentsOfImmuneSystem.htm 

Basic Principles in Immunology & Pathology
http://www.niaid.nih.gov/final/immun/immun.htm

Oddly enough, infection with parasitic worms may reduce the risk of asthma:
http://facstaff.cbu.edu/~seisen/TheWormHasTurned.htm  

 

 

3

9/5

F

Phylum Arthropoda: Form, Function and Classification
http://facstaff.cbu.edu/~seisen/Arthropoda_files/frame.htm 

Crustaceans

Cymothoa exigua is a crustacean which causes the tongue of a fish to atrophy and fall off.(!):

http://facstaff.cbu.edu/~seisen/Cymothoa_exigua.htm

http://www.youtube.com/watch?v=327-bwMQI-Y&feature=youtu.be

33


34

149-181

9/8

M

Class Insecta
http://facstaff.cbu.edu/~seisen/ClassInsecta.htm 

Insect life cycles
http://www.kendall-bioresearch.co.uk/life.htm

Insect development
http://www.cals.ncsu.edu/course/ent425/tutorial/morphogenesis.html

 

 



33

 

 

 

 

 


149-181

9/10

W

Labor Day – No Class

 

 

9/12

F

Mallophaga & Anoplura

·         Pediculus humanus

http://www.youtube.com/watch?v=5a3_SZtgHYE

·         Phthirus pubis

Phthirus pubis on eyelashes:

http://www.youtube.com/watch?v=TpKvYtLxvvI

Hemiptera: True bugs

·         Cimex lectularis

·         Rhodnius prolixus

·         Triatoma infestans

36

 

 

 

 

 

37

168-174

 

 

 

 

161-163

9/15

M

Siphonoptera: The fleas

·         Pulex irritans

·         Ctenocephalides spp.

·         Xenopsylla cheopis

·         Tunga penetrans

38

164-167

9/17

W

What’s causing that itch?  (A slideshow from Medscape):
http://www.medscape.com/features/slideshow/bedbugs-lice-mites?src=mp&spon=17&uac=40240FX

 

 

9/19

F

LECTURE EXAM I – will include Chapters 1 and 2 from Parasite Rex.   

 

 

9/22

M

Diptera: Family Culicinae (Mosquitos)

Presentation on the biology of the Family Culicinae

Presentation on the history of the yellow fever outbreaks in Memphis, and their effect on the local population:  A review of Crosby (2007).  An American Plague. 

39

154-160

9/24

W

Diptera: Other flies

·         Simulium spp.

·         Glossina spp.

·         Lutzomyia spp.

·         Chrysops spp.

·         Phlebotomus spp.

·         Botflies:  http://ambergriscaye.com/pages/town/botfly.html

·         Cuterebra:  http://entnemdept.ufl.edu/creatures/misc/flies/squirrel_bot_fly.htm

39

154-160

9/26

F

Rosh Hashanah – 2nd day

 

 

9/29

M

Hymenoptera

·         Copidosoma floridanum

·         Infected caterpillar becomes a “nursery” for parasitic wasps:  http://www.youtube.com/watch?v=vMG-LWyNcAs&feature=related

·         The wasp Ampulex compressa is a parasitoid, whose females inject venom to lay an egg into the cockroach Periplaneta americana -- “Zombie” cockroach -- http://www.youtube.com/watch?v=DzGCSk1Zpoo

·         Jewel wasp paralyzing a cockroach, laying its egg, and barricading it in a laboratory setting:  http://www.youtube.com/watch?v=qN2XMyxAs5o 

·         Cicada-killer trying to subdue a cicada:
http://www.youtube.com/watch?v=8yjrwkTpAtc

40

 

10/1

W

Basic Principles of Epidemiology
http://facstaff.cbu.edu/~seisen/AnIntroductionToEpidemiology_files/frame.htm

4

 

118-119

 

10/3

F

Parasitic Arachnids: Ticks and Mites

"Everything you never wanted to know about the mites that eat, crawl, and have sex on your face" -- http://blogs.discovermagazine.com/notrocketscience/2012/08/31/everything-you-never-wanted-to-know-about-the-mites-that-eat-crawl-and-have-sex-on-your-face/

·         Dermacentor andersoni

·         Ixodes spp.

·         Trombicula alfreddugesi

·         Demodex spp. -- http://www.youtube.com/watch?v=4IoWb2bUqmk

·         Sarcoptes scabiei

41

175-181

10/6

M

Introduction to the Phylum Platyhelminthes
http://facstaff.cbu.edu/~seisen/Platyhelminthes.htm 

Aspidobothrea

Trematoda: Form, Function, and Classification of Digeneans

Digeneans: Strigeiformes (The schistosomes)

Granuloma Formation by Schistosoma
http://facstaff.cbu.edu/~seisen/GranulomaFormation.htm

13

14

 

15

      16

54-56

 

 

56-77

10/8

W

Digeneans:

·         Fasciola hepatica:  http://facstaff.cbu.edu/~seisen/FasciolaHepatica.htm  

·         Fasciolopsis buski:  http://facstaff.cbu.edu/~seisen/FasciolopsisBuski.htm

·          Paragonimus westermani:  http://facstaff.cbu.edu/~seisen/ParagonimusWestermani.htm   

·         Clonorchis sinensis:  http://facstaff.cbu.edu/~seisen/ClonorchisSinensis.htm   

17

18

56-77

10/10

F

 Sukkot – 2nd day

 

 

10/13

M

Parasites of centrarchids collected from Beaver Lake:

http://facstaff.cbu.edu/~seisen/ParasitesOfCentrarchids.htm

 

 

10/15

W

Lecture Exam 2

 

 

10/17

F

Simchat Torah

 

 

10/20-10/24

M-Su

FALL BREAK

 

 

10/27

M

Parasitism as a driving force for sex, or, The Red Queen Hypothesis:

http://www.indiana.edu/~curtweb/Research/sex&recomb.html

Curtis Lively’s home page:

http://www.indiana.edu/~curtweb/

Endosymbiosis:

http://www.biology.iupui.edu/biocourses/N100/2k2endosymb.html

Monogenea

 

 

 

 

 

 

 

 

 

 

 

 

 

     19

 

 

 

 

 

 

 

 

 

 

 

 

 

78-100

10/29

W

Cestoidea:  Form, Function and Classification of the Tapeworms:

 

http://facstaff.cbu.edu/~seisen/Cestoda.htm

 

Tapeworms: 

·         Diphllobothrium latum – http://facstaff.cbu.edu/~seisen/DiphyllbothriumLatum.htm   

·         Taenia solium – http://facstaff.cbu.edu/~seisen/TaeniaSolium.htm 

·         Taeniarhynchus saginata – http://facstaff.cbu.edu/~seisen/TaeniarhynchusSaginata.htm  

20

 

21

 

 

78-100

10/31

F

Tapeworms, continued:

·         Echinococcus granulosus – http://facstaff.cbu.edu/~seisen/EchinococcusGranulosus.htm 

·         Hymenolepis diminuta -- http://facstaff.cbu.edu/~seisen/HymenolepisDiminuta.htm

·         Vampirolepis nana – http://facstaff.cbu.edu/~seisen/VampirolepisNana.htm 

·         Dipylidium caninum -- http://facstaff.cbu.edu/~seisen/DipylidiumCaninum.htm 

 

78-100

1/3

M

Phylum Nematoda:  http://facstaff.cbu.edu/~seisen/Nematoda_files/frame.htm  

Oddly enough, infection with parasitic worms may reduce the risk of asthma:
http://facstaff.cbu.edu/~seisen/TheWormHasTurned.htm    

Nematoda:  Trichurida and Dioctophymatida

·         Trichuris trichiura –  http://facstaff.cbu.edu/~seisen/TrichurisTrichiura.htm  

·         Trichinella spiralis –

·         http://facstaff.cbu.edu/~seisen/TrichnellaSpiralis.htm

22

 

 

23

101-132

 

 

11/5

W

Nematoda:  Rhabditida, Pioneering Parasites

·         Strongyloides stercoralis

Nematoda:  Strongylidae, Bursate Rhabditians, aka hookworms

·         Ancyclostoma duodenale

·         Ancylostoma caninum

·         Necator americanus

24

25

101-132

11/7

F

Nematodes:  Ascaridida, Intestinal Roundworms

·         Ascaris lumbricoides:  http://facstaff.cbu.edu/~seisen/AscarisLumbricoides.htm  

·         Toxocara

Nematoda:  Oxyurida, the Pinworms

Enterobius vermicularis:  http://facstaff.cbu.edu/~seisen/EnterobiusVermicularis.htm 

26

27

101-132

11/10

M

Nematoda:  Spirurida, a Potpourri

Nematodes:  (Filaroidea), including Wuchereria, Onchocerca and Dirofilaria

·         Wuchereria bancrofti:  http://facstaff.cbu.edu/~seisen/WuchereriaBancroft.htm

·         Onchocerca volvulus:  http://facstaff.cbu.edu/~seisen/OnchocercaVolvulus.htm

·         Loa loa

·         Dirofilaria immitis:  http://facstaff.cbu.edu/~seisen/DirofilariaImmitis.htm

Maybe you shouldn’t go here:

http://facstaff.cbu.edu/~seisen/mammaryeleph.jpg

Maybe you shouldn’t go here either:

http://facstaff.cbu.edu/~seisen/eleph.fijiEN.gif

Biology as art, or maybe it’s art as biology:

http://facstaff.cbu.edu/~seisen/doorknocker.jpg

Nematodes, the Guinea Worms & Others

·         Dracunculus medinensis:  http://facstaff.cbu.edu/~seisen/DracunculusMedinensis.htm 

28

29


30

Please read pdf file:  Ivermectin & River Blindness. pdf

101-132

11/12

W

Phylum Acanthocephala:

http://facstaff.cbu.edu/~seisen/PhylumAcanthocephala.htm

Polymorphus paradoxus:

http://facstaff.cbu.edu/~seisen/PolymorphusParadoxus.htm

Parasites Can Alter Host Behavior
http://facstaff.cbu.edu/~seisen/ParasitesAlteringHostBehavior.htm

32

141-146

11/14

F

Lecture Exam III

 

11/17

M

Miscellaneous invertebrate phyla:

Phylum Mollusca: 

http://facstaff.cbu.edu/~seisen/PhylumMollusca.htm

Natural fish lure -- Lampsilis and bass

http://www.youtube.com/watch?v=I0YTBj0WHkU

Snuffbox Mussel pumping glochidia into gills of fish

http://www.youtube.com/watch?v=0V6V4daH_Ik

Annelida--

http://facstaff.cbu.edu/~seisen/PhylumAnnelida.htm

Hirudo medicinalis:

http://facstaff.cbu.edu/~seisen/HirudoMedicinalis.htm  

Medicinal leeches

http://www.youtube.com/watch?v=ZqgupBRIYps

A British journalist allows himself to be bled

http://www.youtube.com/watch?v=eA40qzrLhqc&feature=related

Tyrannobdella rex  

 

147-148

11/19

W

Parasitic Chordates (brood parasitism among birds, lamprey)

Brood Parasitism—

http://facstaff.cbu.edu/~seisen/BroodParasitism.htm

Nature of the cuckoo duck – David Attenborough – BBC wildlife

http://www.youtube.com/watch?v=4Mb0GOITRUU&feature=fvwe2  

A parasitic cuckoo can be a life saver:

https://www.sciencenews.org/article/parasitic-cuckoo-can-be-good-thing

Petromyzon marinus – the lamprey

http://facstaff.cbu.edu/~seisen/PetromyzonMarinus.htm

Invading Species Awareness PSA - Sea Lamprey

http://www.youtube.com/watch?v=x-KJZ22-wTQ

Sea lamprey spawning:

http://www.youtube.com/watch?v=dHmesdUKDUs&feature=related

Lamprey eliminator:

http://www.youtube.com/watch?v=eqoT8tcZq6k&feature=related

Path of sea lamprey through great lakes

http://dnr.wi.gov/fish/lakesup/images/image003.jpg

The DREADED Candiru

http://facstaff.cbu.edu/~seisen/TheDreadedCandiru.htm

 

182-186

11/21

F

Parasitic Protistans: Form, Function and Classification
http://facstaff.cbu.edu/~seisen/ParasiticProtozoa.pptx

Phylum Sarcomastigophora   

Order Kinetoplastida: The trypanosomes http://www.medicalecology.org/diseases/d_african_trypano.htm

Morphological plasticity of kinetoplasts:

http://upload.wikimedia.org/wikipedia/commons/2/29/TrypanosomatidMorphologies_PlainSVG.svg

·         African trypanosomiasis:  Trypanosoma rhodesiense, T. gambiense, T. brucei

·         American trypanosomiasis:  Trypanosoma cruzi

Presentation on the rise of Chagas’ Disease in the continental United States

4

14-53

11/24

M

Order Kinetoplastida: The leishmanias

·         Leishmania donovani

·         Leishmania tropica

·         Leishmania braziliensis

5

 

29-36

 

11/26

W

Other Flagellates

·         Chilomastix mesnili

·         Giardia lamblia

·         Trichomonas vaginalis

6

24-28

11/27

Th

Thanksgiving Holiday

 

 

11/28

F

Thanksgiving Holiday

 

 

12/1

M

Subphylum Sarcodina: The Amebas

·         Entamoeba histolytica

·         Entamoeba coli

·         Entamoeba gingivalis

·         Endolimax nana

·         Iodamoeba butschlii

·         Naegleria fowleri

·         Acanthamoeba castellanii

7

15-23

12/3

W

Phylum Apicomplexa: Gregarines, Coccidia, and Related organisms:

http://www.tulane.edu/~wiser/protozoology/notes/api.html

·         Toxoplasma gondii

·          Pneumocystis

·         Cryptosporidium)

8

37-50

12/5

F

LECTURE EXAM IV     

 

 

12/8

M

Phylum Apicomplexa: Malaria Organisms and Piroplasms (Plasmodium spp.)

http://facstaff.cbu.edu/~seisen/Malaria.htm 

A description of sickle cell anemia:  etiology, pathology, treatment
http://emedicine.medscape.com/article/205926-overview?src=emed_whatnew_nl_0#growth

9

37-50

12/10

W

Presentation on  drug resistance in malaria, and current status of drug development

Presentation on current status of vaccine development for malaria 

Presentation on genetic responses to malaria

Phylum Ciliophora: Ciliated Protistan Parasites (Ichthyophthirus multifiliis, Balantidium coli)

Phyla Myxozoa and Microspora: Protozoa with Polar Filaments

 

 

 

 

10

11

 

 

 

 

51-53

12/12

F

Plants

Mistletoe – We kiss under this?

http://www.youtube.com/watch?v=89gdG6x3cog

http://facstaff.cbu.edu/~seisen/ParasiticPlants.htm 

 

187-201

12/15-19

M-F

***Final Exam Week***

Tentative Lab Schedule: Fall 2013

Date

EXERCISE

PAGE(S) in Lab book

Tue.,  Aug. 20

Studies in Mutualism

Insects which induce gall formation in plants

203-206

244-246

 Tue., Aug. 27

Begin mosquito bioassay experiment

Insects:
Order Diptera
Culex spp.

·         Ova

·         Larvae

·         Pupae

·         Adult males

·         Adult females

Glossina spp.

·         Adults

231-234

154-160

 

 Tue., Sept. 3

Finish mosquito bioassay experiment data collection

Phylum Arthropoda

Class Crustacea

Argulus sp. (fish louse)

adult, w.m.

 

Argulus:

http://www.youtube.com/watch?v=2vPwaDtMYrs

 

 

Class Insecta

Order Anoplura

Pediculus humanus (body louse)

Phthirus pubis (public louse)

Order Siphonoptera

Pulex irritans (human flea)

Ctenocephalides canis (dog flea)

231-234

 

 

168-174

 

 

 

 

164-168

 Tue., Sept. 10

Order Hemiptera

Cimex lectularis (bedbug)

Adults, w.m.

 

Arachnids:
Dermacentor andersoni

·         Adult females

·         Adult males

·         Larvae

Ixodes spp.

·         Adults

Family Trombiculidae

·         Adult from Rana pipiens

Techniques in preparing permanent slides

161-163

 

 

 

 

 

 

 

175-181

 

 

 

 

 

 

 

 

 

 

 

 

 

217-222

 Tue., Sept. 17