Instructor: Dr. Dennis L. Merat
Office Hours: Room S302B
9:10 a.m. – 10:50 a.m. MTWF
3:15 p.m. – 5:00 p.m. M
11:00 a.m. – 11:50 p.m. T
1:00 p.m. – 1:50 p.m. T
Phone Numbers: 321-4201 (Office)
362-3682 (Home, 8 p.m. - 10 p.m.)
E – mail: firstname.lastname@example.org
Course Description: This course is designed to accompany Biochemistry I. It will provide an introduction to laboratory techniques used in the isolation and characterization of the major classes of biological molecules. Emphasis will be placed on techniques for protein purification and assay including chromatography, electrophoretic methods, centrifugation, spectroscopy, and enzyme kinetics. Bioinformatics will be discussed throughout the course.
Prerequisite or Corequisite: Chem 315
Required Texts and Materials:
1. Biochemistry Laboratory, by Rodney
Boyer, Benjamin Cummings,
2. Scientific Calculator
General Course Goals: The course is designed to:
1) Introduce students to the basic techniques
and procedures used in the biochemistry laboratory including: spectrophotometry,
chromatography, centrifugation, and enzyme kinetics;
2) Illustrate principles discussed in Chemistry 315 .
Attendance: Regular laboratory attendance is required.
6 laboratory reports @ 100
points each 600 points
Homework 100 points
Mid-term Examination 75 points
Comprehensive Laboratory Final 150 points
Average (%) Letter Grade
90 -- 100
80 -- 89 B
65 -- 79 C
54 -- 64 D
53 or below F
The student’s average for this course is calculated by dividing the total points earned by 925, then multiplying by 100. If your scores are within these ranges, you are guaranteed the appropriate letter grade for the range within which your scores fall.
For problems involving numeric calculations, ALL WORK, except simple arithmetic, must be shown to receive any credit. Partial credit will be given.
Each homework assignment will be graded on a 100 point scale; the homework grade for the semester will be computed using the following formula:
Sum of numerator homework scores/total number assignments
Make-up and Late Policy: Late assignments will be accepted only under the most extenuating circumstances and may receive lower grades than assignments turned in on time. Labs may be made up only under very extenuating circumstances.
Mid-Term Examination: A mid-term examination will be given on October 12, 2005 .
Final Examination: A comprehensive final examination will be given
during the final examination period at a time to be announced later in
Experiments to be performed this semester include:
pH and Buffers
Paper Chromatography/Identification of Unknown Amino Acids
Gel Filtration: Separation of BSA from Phenol Red
Introduction to Spectrophotometry/Protein Determinations
SDS Electrophoresis/Molecular Weight Determination
Separation of Proteolytic Digests
Electrophoresis and Identification of Phosphoproteins
Inversion of Sucrose
Measurement of Cholesterol and Vitamin C in Biological Samples
Isolation and Characterization of DNA